This single-center, non-comparative, randomized, matched pilot study investigated the levels of cutaneous microorganisms before and after antiseptic application using both techniques in a population of healthy French volunteers.
Hence, this study conducted this comparison.
The two techniques most commonly used to apply antiseptic to the skin are the concentric circle and back-and-forth techniques, but these have not been compared in clinical trials. There is no consensus on the best way to clean the skin prior to catheter insertion, which could be a key element of CRBI prevention. However, a common complication associated with these is catheter-related bloodstream infection (CRBI) due to the introduction of microorganisms from the skin during catheter insertion. In France about 32% of hospitalized patients have a vascular access placement. Diverse clonal relationship between the strains was identified in Staphylococcus epidermidis.ĭetection of contaminants associated with environmental and skin flora, shows the need to implement measures in the process of disinfecting skin at the site of phlebotomy and cleaning each of the areas involved in blood collection.
Nine of the 100 PC analyzed (9%) showed bacterial contamination analysis of the nucleotide sequences revealed the presence of characteristic microbiota from donor skin and soil.
Additionally, molecular biology tests were implemented in order to identify the possible contamination sources. This sample size corresponds to 1% of the PC obtained by the National Center of Blood Transfusion (NCBT) in Mexico City according to the Official Mexican Standard NOM-253-SSA1-2012.
One hundred PC were randomly selected and microbiologically analyzed. In Mexico, the identification and molecular characterization of bacterial contaminants to detect contamination sources have not been implemented due to high costs however, it is an actual current need. The screening of PC to detect bacterial contamination is obligatory in Mexico, and it is carried out in quality control programs. Most common bacterial sepsis associated with transfusion is caused by contaminated Platelet Concentrates (PC). This necessitates its usage in all other blood banks of Pakistan to enhance the safety of transfused blood. Conclusion: The results of this study suggest the significance of diversion as an effective way of preventing bacterial contamination of whole blood components. The platelet component of the positive whole blood sample showed an absence of the contaminant. The bacterial species identified were coagulase-negative Staphylococcus. Results: The prevalence of bacterial contamination of whole blood using the diversion pouch was detected as 0.3% (n = 1/300). The culture vials flagged positive were subcultured onto a set of solid media to isolate and identify the implicated microbial species, followed by testing the whole blood-derived platelet component of the blood sample that tested positive. Aerobic and anaerobic blood culture vials were inoculated and incubated in the BD BACTEC™ FX40 instrument at 35☌ until positive and for 7 days if negative. A total of 300 diversion pouches filled with 20 mL of whole blood samples were included in the study.
Methods: A cross-sectional study was performed from December 2019 to February 2020. The aim of this study was to evaluate the prevalence of bacterial contamination and assessing the preventive effect of diversion pouches implemented during whole blood collections in a regional blood center in Rawalpindi, Pakistan. Adhering to stringent blood donor disinfection technique in conjunction with first aliquot diversion has mitigated the risks of bacterial sepsis. Background and Objectives: Blood transfusion is a lifesaving therapeutic intervention however, transfusion-transmissible bacterial infections pose a great risk to recipients.